Cloned (Comment) | Organism |
---|---|
gene FN3K, located on chromosome 17q25.3, genotyping | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | analysis of the FN3K gene on a Caucasian cohort of diabetic patients, molecular characterization of the FN3K gene by analyzing its promoter and of polymorphisms, c-385A/G (rs3859206) and the c-232A/T (rs2256339), associated with FN3K enzymatic activity in erythrocytes, as well as two variants c-421C/T and c-429delATCGGAG | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxymorpholinofructose | a competitive inhibitor | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-N6-D-fructosyl-L-lysine | Homo sapiens | - |
ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9H479 | gene FN3K | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
erythrocyte | - |
Homo sapiens | - |
lens | - |
Homo sapiens | - |
additional information | FN3K is more active in tissues containing proteins with long (half-)lives, such as erythrocytes, lens and brain | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-N6-D-fructosyl-L-lysine | - |
Homo sapiens | ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine | - |
? | |
additional information | the fructosamines bound to Lys139alpha, located near the C-terminus of the alpha subunits, and Lys16alpha, located on a loop of the alpha subunits, are good substrates. The N-terminal glycated valine is a poor substrate, consistent with free fructosevaline being a much poorer substrate than free fructoselysine | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FN3K | - |
Homo sapiens |
fructosamine 3-kinase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the FN3K gene may have arisen by an event of duplication of an ancestral gene, FN3K-related protein (FN3K-RP). The gene encoding FN3K-RP is located next to the one encoding FN3K, and share a 65% sequence homology with FN3K and an identical genome organization. Both FN3K and FN3K-RP phosphorylate psicosamines and ribulosamines, but only the former act on fructosamines | Homo sapiens |
metabolism | starvation and diabetes do not change the level of expression of FN3K in different tissues, and no regulation of FN3K expression is observed in human fibroblasts treated with condition mimicking the diabetic state | Homo sapiens |
physiological function | fructosamine 3-kinase (FN3K) is involved in protein deglycation FN3K phosphorylates fructosamines on the third carbon of their sugar moiety, making them unstable and causing them to detach from proteins, suggesting a protective role of this enzyme. FN3K is able to break down the second intermediate of the non-enzymatic glycation cascade by phosphorylating fructoselysine to a fructoselysine-3-phosphate. The variability in FN3K activity is associated with some polymorphisms in the FN3K gene, FN3K involvement in diabetes, overview. FN3K might act in concert with other molecular mechanisms and may impact on gene expression and activity of other enzymes involved in deglycation process | Homo sapiens |